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Interferon (IFN) and inducible proteins are known to be the initial molecules in the antiviral defence system of the body. In this study, we measured the levels of IFN-alpha, IFN-beta, and p42 in the plasma of patients by using polyclonal antibodies. The plasma levels of IFN-alpha, IFN-beta and p42 were significantly higher in patients with herpes simplex virus type 1 (HSV-1) infection, parvovirus B19 (B19V) infection, cytomegalovirus (CMV) infection, and in recovered patients from CMV infection, compared with healthy controls. The levels of IFN-alpha and p42 in the plasma correlated with the number of viral antigens in the serum of the patients. The plasma level of IFN-alpha and IFN-beta was higher in IFN-alpha/beta receptor-deficient (IFN-alpha/betaR-d/d) mice infected with HSV-1, than in wild-type (WT) mice. The levels of IFN-alpha, IFN-beta and p42 in the plasma correlated with the number of viral antigens in the brain, liver and kidney. 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